Techniques for assessment of teratologic effects: embryo culture.

I-9: Embryo Assessment and Selection

Over the years it has been an important goal to optimize the treatment of subfertility in order to obtain a better pregnancy rate and baby-take-home rate, usually combined with the goal of decreasing the rate of multiplet pregnancies. This implies optimizing the development of embryos through improved in vitro culture methods. And it also implies optimizing the methods of selection of embryos f...

Pharmacokinetic determinants of embryotoxicity in rats associated with organic acids.

We have studied four organic acids of similar structure to further understand the basis of their developmental toxicity. Valproic acid (2-propyl pentanoic acid), ethylhexanoic acid, and octanoic acid are isomeric C8 organic acids but their teratologic potency varied widely. Valproic acid induced a moderate to severe teratologic outcome after a single oral administration of 6.25 mmoles/kg on day...

Inm-1: Blastocyst Culture: Advantages and Disadvantages

Both cleavage-stage and blastocyst-stage embryo transfer policieshave advantages and drawbacks.Potential advantages of blastocyst culture and transfer in human IVF include: i. synchronization of the embryo with the female tract leading to increasedimplantation rates; thereby, reducing the need for multipleembryo transfers, ii. assessment of viability of an embryobefore transfer. This can be ach...

Interactive Effects of Heat Shock and Culture Density on Embryo Induction in Isolated Microspores Culture of Brassica napusL. cv. Global

Embryo Culture Challenge: Microbial Contamination

The success rate of embryo production critically depends on several factors. One of the pivotal factors is a technique used for the whole procedure of in vitro fertilization (IVF) and in vitro embryo culture (IVC) (1, 2). Besides the technique selected for IVF / in vitro embryo production (IVEP), there is an important consideration called IVF/IVEP culture dishes disin...